H2N2V1, Main, Exploration, bibRecord, 002E46

[A method of competitive dot hydridization for genotyping influenza A viruses].

Identifieur interne : 002E46 ( Main/Exploration ); précédent : 002E45; suivant : 002E47

[A method of competitive dot hydridization for genotyping influenza A viruses].

Auteurs : D V Golubev ; F N Zolotarev

Source :

Voprosy virusologii [ 0507-4088 ]

RBID : pubmed:8284914

Descripteurs français

KwdFr :
- ADN viral (génétique), ARN viral (génétique), Animaux, Embryon de poulet, Gènes viraux (génétique), Génotype, Hybridation d'acides nucléiques (), Hybridation d'acides nucléiques (instrumentation), Immunotransfert (), Immunotransfert (instrumentation), Mathématiques, Plasmides (génétique), Sensibilité et spécificité, Virus de la grippe A (), Virus de la grippe A (génétique).
MESH :
- génétique : ADN viral, ARN viral, Gènes viraux, Plasmides, Virus de la grippe A.
- Animaux, Embryon de poulet, Génotype, Hybridation d'acides nucléiques, Immunotransfert, Mathématiques, Sensibilité et spécificité, Virus de la grippe A.

English descriptors

KwdEn :
- Animals, Chick Embryo, DNA, Viral (genetics), Genes, Viral (genetics), Genotype, Immunoblotting (instrumentation), Immunoblotting (methods), Influenza A virus (classification), Influenza A virus (genetics), Mathematics, Nucleic Acid Hybridization (instrumentation), Nucleic Acid Hybridization (methods), Plasmids (genetics), RNA, Viral (genetics), Sensitivity and Specificity.
MESH :
- chemical , genetics : DNA, Viral, RNA, Viral.
- classification : Influenza A virus.
- genetics : Genes, Viral, Influenza A virus, Plasmids.
- instrumentation : Immunoblotting, Nucleic Acid Hybridization.
- methods : Immunoblotting, Nucleic Acid Hybridization.
- Animals, Chick Embryo, Genotype, Mathematics, Sensitivity and Specificity.

Abstract

A new method for genetic typing of influenza viruses using molecular hybridization of DNA-RNA was developed which consisted in addition to the hybridization solution, apart from the radioactively labeled probe, of RNA of a virus with known gene homologous to the plasmid DNA used as the probe but belonging to a different serosubtype of influenza A virus, other than cloned kDNA, (within the range of H1N1, H2N2, and H3N2). This competitive RNA (RNAc) is added in considerable excess with regard to both molecular probe and to RNA immobilized on the filter. Therefore hybrids of molecular probe DNA with RNAc are rapidly formed from which RNAc may be replaced only by those bRNA immobilized on the filter whose homology to the probe is higher than that of RNAc.

PubMed: 8284914

Affiliations:

Le document en format XML

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<author><name sortKey="Golubev, D V" sort="Golubev, D V" uniqKey="Golubev D" first="D V" last="Golubev">D V Golubev</name>
</author>
<author><name sortKey="Zolotarev, F N" sort="Zolotarev, F N" uniqKey="Zolotarev F" first="F N" last="Zolotarev">F N Zolotarev</name>
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<term>Genotype</term>
<term>Immunoblotting (instrumentation)</term>
<term>Immunoblotting (methods)</term>
<term>Influenza A virus (classification)</term>
<term>Influenza A virus (genetics)</term>
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<term>Nucleic Acid Hybridization (methods)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term>
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<term>Virus de la grippe A (génétique)</term>
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<term>RNA, Viral</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Viral</term>
<term>Influenza A virus</term>
<term>Plasmids</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term>
<term>ARN viral</term>
<term>Gènes viraux</term>
<term>Plasmides</term>
<term>Virus de la grippe A</term>
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<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Immunoblotting</term>
<term>Nucleic Acid Hybridization</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunoblotting</term>
<term>Nucleic Acid Hybridization</term>
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<term>Chick Embryo</term>
<term>Genotype</term>
<term>Mathematics</term>
<term>Sensitivity and Specificity</term>
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<term>Génotype</term>
<term>Hybridation d'acides nucléiques</term>
<term>Immunotransfert</term>
<term>Mathématiques</term>
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<front><div type="abstract" xml:lang="en">A new method for genetic typing of influenza viruses using molecular hybridization of DNA-RNA was developed which consisted in addition to the hybridization solution, apart from the radioactively labeled probe, of RNA of a virus with known gene homologous to the plasmid DNA used as the probe but belonging to a different serosubtype of influenza A virus, other than cloned kDNA, (within the range of H1N1, H2N2, and H3N2). This competitive RNA (RNAc) is added in considerable excess with regard to both molecular probe and to RNA immobilized on the filter. Therefore hybrids of molecular probe DNA with RNAc are rapidly formed from which RNAc may be replaced only by those bRNA immobilized on the filter whose homology to the probe is higher than that of RNAc.</div>
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<name sortKey="Zolotarev, F N" sort="Zolotarev, F N" uniqKey="Zolotarev F" first="F N" last="Zolotarev">F N Zolotarev</name>
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[A method of competitive dot hydridization for genotyping influenza A viruses].

[A method of competitive dot hydridization for genotyping influenza A viruses].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration H2N2
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